Background:

The p21 activated kinases (PAK) are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. The PAK proteins are a family of serine/threonine kinases that serve as targets for the small GTP binding proteins, CDC42 and RAC1, and have been implicated in a wide range of biological activities. The protein encoded by this gene is activated by proteolytic cleavage during caspase-mediated apoptosis, and may play a role in regulating the apoptotic events in the dying cell.

Applications:

WB

Name of antibody:

PAK2(Phospho-Ser141)

Immunogen:

Synthetic peptide of human PAK2

Full name:

p21 (RAC1) activated kinase 2

Synonyms:

PAK65; PAKgamma

SwissProt:

Q13177

WB Predicted band size:

58 kDa

WB Positive control:

HepG2 cells treated with Adriamycin (0.5uM, 24hours) and Jurkat cells treated with PMA (125ng/ml, 30mins)

WB Recommended dilution:

500-1000