Background:

Antenna protein that modulates the color of the bioluminescence emission of the luciferase. In the presence of YFP and only at temperatures below 20 degrees Celsius, luciferase exhibits a bimodal emission spectrum with a new peak at 545 nM (yellow), in addition to the one at 485 nM.

Applications:

WB, IF, FC

Name of antibody:

tYFP

Immunogen:

Purified tYFP protein expressed in HEK293T cell

SwissProt:

P21578

WB Predicted band size:

22 kDa

WB Positive control:

HEK293T cells were transfected with the pCMV6-ENTRY control (left lane)or pCMV6-ENTRY tYFP ne) cDNA for 48 hrs and lysed

WB Recommended dilution:

500-2000

IF Positive control:

COS7 cells transiently transfected by pCMV6-ENTRY

IF Recommended dilution:

50-100

FC Recommend dilution:

200-400